RBS

Part:BBa_J119053:Design

Designed by: David Carr   Group: Eckdahl_Lab   (2012-01-25)


J23100 Promoter with BD18 RBS w/ leader and RFP reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 850
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 39
    Illegal NheI site found at 62
    Illegal SpeI site found at 851
    Illegal PstI site found at 865
    Illegal NotI site found at 7
    Illegal NotI site found at 858
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 851
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 851
    Illegal PstI site found at 865
    Illegal AgeI site found at 723
    Illegal AgeI site found at 835
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter has two BsaI sites, one at each end of the sequence. The one on the 5' end of J23100 cuts to the left, and the one at the 3' end of J23100 cuts to the right. There are also two BbsI sites, one is up stream from the 5' BsaI site and cuts to the left, while the other is down stream from the 3' BsaI site and cuts to the right.


Source

de novo, selected sequence

References